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Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

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  • 产品名称:Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)
  • 产品型号:PS2-FTDB
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简单介绍

Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO) DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

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Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

名称

货号

英文名、缩写

分子量

规格

价格

FITC-PEG-DBCO

PS2-FTDB-5K

FITC-PEG-DBCO

5000

0.1g0.5g

现询

FITC-PEG-DBCO

PS2-FTDB-2K

FITC-PEG-DBCO

2000

0.1g0.5g

现询

FITC-PEG-DBCO

PS2-FTDB-3K

FITC-PEG-DBCO

3400

0.1g0.5g

现询

FITC-PEG-DBCO

PS2-FTDB-1K

FITC-PEG-DBCO

1000

0.1g0.5g

现询

FITC-PEG-DBCO

PS2-FTDB-10K

FITC-PEG-DBCO

10000

0.1g0.5g

现询

(备注:非常规分子量:400600。**聚合度的小分子定制。)

结构式:

=========================================

 

基本信息:

========================================= 

中文名称:Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

别名:FITC-PEG-DBCO

英文名称:FITC-PEG-DBCO,Fluorescein-PEG-DBCO

CAS:-

分子量:400、600、1K、2K、3.4K、4K、5K、10K

纯度:90%以上

 

应用简介:

=========================================

DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.


物理性质:

========================================= 

状态:淡黄色到黄色固体,或者半固体,取决于分子量。

溶解性:溶于DMSO,DMF等溶剂。

保持条件:-20℃长期保存,避光,干燥。

注意事项:取用一定要干燥,避免频繁的溶解和冻干。

反应性:DBCO于含有N3基团的反应。


技术指标:

========================================= 

纯度(PEG):90%以上

取代率(DBCO):90%以上

取代率(FITC):90%以上

分散系数(PDI):小于等于1.05

分析:COA、GPC、NMR、MALDI-TOF

Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)

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